5x HOT FIREPol® Probe Universal qPCR Mix is optimized for
real-time quantitative PCR assays and contains all the components necessary
to perform qPCR, with the exception of template, primers, and probe. The qPCR
Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in
a proprietary reaction buffer that enables efficient amplification of
AT-rich, regular and GC-rich (up to 75%) targets. HOT FIREPol® Probe
Universal qPCR Mix is optimized for DNA/LNA hydrolysis probes based on the
5´–3´ exonuclease activity.
HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at
95°C. This prevents extension of non-specifically annealed primers and
primer-dimers formed at low temperatures during qPCR setup.
